biotin mouse anti human il 1β detection antibody (R&D Systems)
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Biotin Mouse Anti Human Il 1β Detection Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+mouse+anti+human+il+1%CE%B2+detection+antibody/pmc10982322-69-16-22?v=R%26D+Systems
Average 96 stars, based on 935 article reviews
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1) Product Images from "The monocyte-derived cytokine response in whole blood from preterm newborns against sepsis-related bacteria is similar to term newborns and adults"
Article Title: The monocyte-derived cytokine response in whole blood from preterm newborns against sepsis-related bacteria is similar to term newborns and adults
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2024.1353039
Figure Legend Snippet: The production of TNF-α and IL-6 by term neonatal and adult WB shows a high degree of inter-individual variation. WB from term cord blood (term, N=11-17) and healthy adults (adult, N=11-18) was incubated with (A, B) LPS, Pam3Cys4K or R848 or with (C, D) E. coli , S. agalactiae (GBS), S. aureus or S. epidermidis (CNS) bacteria for 5h and the production of TNF-α and IL-6 was measured. (E) The inter-individual variation in production of TNF-α and IL-6 between individuals was determined for WB from term cord blood and healthy adults (N=11-18) for LPS (100 ng/mL), Pam3Cys4K (10 μg/mL) or R848 (10 μM) or bacteria (10 5 CFU/well). Cytokine concentrations are shown in pg/mL. CFU, colony-forming units.
Techniques Used: Incubation, Bacteria
Figure Legend Snippet: Preterm neonatal WB produces lower levels of IL-6 and similar levels of TNF-α compared to term neonatal WB. Whole blood from preterm cord blood (preterm, N=8-10), term cord blood (term, N=17) and healthy adults (adult, N=17-21) was incubated with TLR ligands LPS (100 ng/mL), Pam3Cys4K (10 μg/mL) or R848 (10 μM) or with E. coli , S. agalactiae (GBS), S. aureus or S. epidermidis (CNS) bacteria (10 5 CFU/well) for 5h and the production of (A, B) TNF-α, (C, D) IL-6 and (E, F) IL-1β was measured. (G-I) The inter-individual variation in production of (G) TNF-α, (H) IL-6 and (I) IL-1β between individuals was determined for preterm cord blood (N=8-10). Cytokine concentrations are shown in log-transformed pg/mL. The dotted line indicates the limit of detection. CV = coefficiency of variation. * = P<0.05. ** = P<0.01. *** = P<0.001.
Techniques Used: Incubation, Bacteria, Transformation Assay